禽腺病毒血清4型感染鸡组织中NLRP3基因转录水平的实时荧光定量PCR分析     

郭慧芳  李宁  王白玉  乔麒龙  黄庆  李永涛  王增  赵军 《畜牧兽医学报》2021,52(1):195-201

旨在分析禽腺病毒血清4型（FAdV-4）感染鸡组织中NLRP3基因的转录水平,本研究设计鸡NLRP3特异性引物,利用RT-PCR扩增NLRP3基因180 bp片段并克隆至pMD-18T载体,制备重组质粒pMD-18T-NLRP3。以pMD-18T-NLRP3质粒作为标准品进行荧光定量PCR并建立标准曲线。通过反应条件优化,成功建立了检测NLRP3基因的实时荧光定量PCR方法,并利用该方法对致病性FAdV-4感染鸡组织中NLRP3基因的转录水平进行了分析。结果显示,所设计的NLRP3引物可特异性扩增鸡NLRP3基因,建立的实时荧光定量PCR对鸡NLRP3标准质粒的扩增曲线良好,标准品的拷贝数与Cq值呈现良好的线性关系。与对照组相比,NLRP3分子在FAdV-4感染鸡肝和脾中的转录水平极显著高于对照组（P<0.001）,在盲肠扁桃体和法氏囊的表达显著高于对照组（P<0.01）。本研究所建立的鸡NLRP3基因SYBR Green Ⅰ实时荧光定量PCR可以检测FAdV-4感染鸡不同组织中NLRP3的转录水平;致病性FAdV-4感染所造成的组织炎症损伤与NLRP3分子密切相关。  相似文献   

2018—2020年湖南省宁乡市牛羊布鲁氏菌病血清学监测     

王文梅  侯禛  曾勇波  胡长青  周元中 《中国动物检疫》2021,38(9):5-8

为掌握宁乡市牛羊布鲁氏菌病防控净化效果,2018—2020年在全市开展了牛羊布鲁氏菌病监测工作。共检测1 932个牛羊养殖场户,采集牛羊血清样本32 356份,应用虎红平板凝集试验(RBPT)进行初筛,竞争酶联免疫吸附试验(c ELISA)进行复核。结果显示:2018—2020年共检出阳性场户10个(羊场户9个、牛场户1个),平均场户阳性率为0.52%;检出阳性样品82份,平均个体阳性率为0.25%。2018年场户阳性率为1.57%,个体阳性率为0.33%,2019年场户阳性率为0.15%,个体阳性率为0.06%;2020年场户阳性率为0.24%,个体阳性率为0.41%。2018年检出阳性乡镇5个,2019年1个,2020年2个。结果表明,宁乡市布鲁氏菌病流行得到有效控制,流行范围大幅缩小,但传播风险依然存在,还需继续加大监测和防控力度,加快推进布鲁氏菌病净化。本研究为宁乡市布鲁氏菌病防控提供了数据支撑。  相似文献   

Effects of soybean raffinose on growth performance,digestibility,humoral immunity and intestinal morphology of growing pigs     

Zhu Zeng  Yalin Zhang  Jun He  Jie Yu  Xiangbing Mao  Ping Zheng  Yuheng Luo  Junqiu Luo  Zhiqing Huang  Bing Yu  Daiwen Chen 《动物营养（英文）》2021,7(2):393

There are appreciable does of raffinose in soybean, but the impacts of raffinose on pigs are poorly investigated. We used 2 experiments to investigate the influence of soybean raffinose on growth performance, digestibility, humoral immunity and intestinal morphology of growing pigs. In Exp. 1, a total of 30 crossbred (Duroc × Landrace × Yorkshire) barrows (21.93 ± 0.43 kg) were randomly divided into 3 groups, and were fed with the control diet, the control diets supplemented with 0.2% and 0.5% raffinose, respectively, for 21 d. Results showed that the addition of 0.2% or 0.5% raffinose reduced (P < 0.05) average daily feed intake (ADFI), average daily gain (ADG) and nutrient digestibility, and dietary 0.5% raffinose increased the ratio of feed to gain (P < 0.05). For serum indexes, dietary 0.5% raffinose decreased growth hormone and increased glucagon-like peptide-2, immunoglobulin G, tumor necrosis factor-α (TNF-α) and interleukin-6 concentration (P < 0.05). In Exp. 2, a total of 24 crossbred barrows (38.41 ± 0.45 kg) were randomly divided into 3 groups, and were fed with the control diet (ad libitum), the raffinose diet (0.5% raffinose, ad libitum), and the control diet in the same amount as the raffinose group (feed-pair group) for 14 d, respectively. Compared with the control diet, dietary 0.5% raffinose decreased ADFI (P < 0.05). Intriguingly, the raffinose group had lower ADG than the feed-pair group, lower nutrient digestibility, lower amylase activity in duodenum, lower amylase, lipase and trypsin activities in jejunum and higher TNF-α concentration in serum compared with the other 2 groups, and a higher ratio of villus height to crypt depth compared with the control group (P < 0.05). These results showed that soybean raffinose could reduce feed voluntary intake and body gain while improving intestinal morphology without a significant negative influence on immunity. Taken together, dietary raffinose could decrease growth performance by reducing both feed intake and nutrient digestibility while inducing humoral immune response of growing pigs.  相似文献   

冬小麦种质“矮孟牛”中新型小麦-黑麦复杂易位的遗传传递分析   总被引：8，自引：3，他引：8  

亓增军  刘大钧  陈佩度  王苏玲  李斯深  李晴祺 《作物学报》2001,27(5):582-589

本文利用矮孟牛中一种新型小麦-黑麦复杂易位IRS·7DS,1BL·7DL与1BL·1RS易位所构建的一套重组自交系(包括109个F6株系)和8个矮孟牛衍生品种进行了分析,结果表明在109个F6株系中,纯合复杂易位占69个,纯合1BL·1RS占36个,二者比例约2∶1,杂合类型占4个,出现频率4/109;对矮孟牛8个衍生品种的分析表明,6个品种包含来自矮孟牛的  相似文献   

农业远程温湿度监测装置的设计—基于NRF24LE1和GPRS     

周小云  曾炼成 《农机化研究》2015,(2):206-209

农产品的质量与其生长环境和保存环境密切相关,而农业对象又具有分散偏僻的特点,因此研究便捷、低功耗、精确的远程温湿度监测装置具有现实意义。此装置由传感节点和中继节点组成,传感节点选用NRF24 LE1低功耗器件,负责感知环境的温湿度,通过2.4 GHz无线短距传输上传至中继节点;终端PC利用GPRS远距离与中继节点通信,实时周期性收集中继节点汇聚的传感数据。传感节点与中继节点间采用防碰撞延时发送机制,实现传感节点的低功耗和数据有效传输。中继节点与终端PC通过GPRS网络建立UDP连接,使系统可以管理多个中继节点,可扩展性好。  相似文献   

The overexpression of cytochrome P450 genes confers buprofezin resistance in the brown planthopper,Nilaparvata lugens (Stål)     

Bin Zeng  Ya-Ting Liu  Ze-Rui Feng  Fu-Rong Chen  Shun-Fan Wu  Chris Bass  Cong-Fen Gao 《Pest management science》2023,79(1):125-133

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An Information System for Vehicle Transport Management     

Zeng Fanqi Zhou Ming Wang Yong 《保鲜与加工》1993,(4):150-154

This paper proposes a model framework, which describes the function requirements and the common characterstics of vehicle transport management. With the frame, a computer vehicle transportation managerment information system(QCMIS)is in troduced here and it has already been put into practice. The key technical points is also explained in the paper.  相似文献   

A STUDY OF THE SENSITIVITY OF BIQUADRATIC SWITCHED CAPACITOR FILTER     

Zeng Xiaoping 《保鲜与加工》1991,(4):46-52

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Detection of Edges within the Cross Sectionfrom Projections of Parallel Beams Via the Wavelet Method     

Zeng Li  Yi Dong  Chen Tinghuai 《保鲜与加工》1996,(6):85-89

The wavelet analysis are used in the detection of edges within the cross sectionfrom projections of parallel beams. This method can be applied in computerized tomography (CT) inorder to reduce the noise and the number of projections.  相似文献   

兰花选育种研究进展   总被引：5，自引：0，他引：5  

曾碧玉  朱根发  刘海涛 《中国农学通报》2005,21(12):272-272

兰科植物为单子叶植物中最大的一个科。全国各地极其重视资源的调查、收集与驯化及其外来品种的引进；已有17个种的兰花种子成功运用超低温技术贮存；染色体分析技术及分子标记成为资源评价的重要手段，应用于研究兰科植物的亲缘关系及其遗传多样性这两方面的研究报道较多。兰花育种以传统的人工杂交为主，通过人工杂交已获得大量种间、属间杂交种；多倍体育种方法及生物技术作为辅助手段应用于兰花育种，获得了多倍体植株及转基因植株。  相似文献